First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey

Enrique Lara; Leonardo D. Fernández; M. Romina Schiaffino; Irina Izaguirre

doi:10.1016/j.ejop.2017.05.008

First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey

Enrique Lara, Leonardo D. Fernández, M. Romina Schiaffino, Irina Izaguirre

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene + ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters.

Original language	English
Pages (from-to)	45-49
Number of pages	5
Journal	European Journal of Protistology
Volume	60
DOIs	https://doi.org/10.1016/j.ejop.2017.05.008
State	Published - Aug 2017
Externally published	Yes

Keywords

Mamiellophyceae
Picoplankton
Ribosomal genes
Southern Patagonia

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10.1016/j.ejop.2017.05.008

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title = "First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey",

abstract = "We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene + ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters.",

keywords = "Mamiellophyceae, Picoplankton, Ribosomal genes, Southern Patagonia",

author = "Enrique Lara and Fern{\'a}ndez, {Leonardo D.} and Schiaffino, {M. Romina} and Irina Izaguirre",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier GmbH",

year = "2017",

month = aug,

doi = "10.1016/j.ejop.2017.05.008",

language = "English",

volume = "60",

pages = "45--49",

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T1 - First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey

AU - Lara, Enrique

AU - Fernández, Leonardo D.

AU - Schiaffino, M. Romina

AU - Izaguirre, Irina

PY - 2017/8

Y1 - 2017/8

N2 - We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene + ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters.

AB - We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene + ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters.

KW - Mamiellophyceae

KW - Picoplankton

KW - Ribosomal genes

KW - Southern Patagonia

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SN - 0932-4739

VL - 60

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JO - European Journal of Protistology

JF - European Journal of Protistology

ER -

First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey

Abstract

Keywords

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