Salmon-IgM Functionalized-PLGA Nanosystem for Florfenicol Delivery as an Antimicrobial Strategy against Piscirickettsia salmonis

Felipe Velásquez, Mateus Frazao, Arturo Diez, Felipe Villegas, Marcelo Álvarez-Bidwell, J. Andrés Rivas-Pardo, Eva Vallejos-Vidal, Felipe Reyes-López, Daniela Toro-Ascuy, Manuel Ahumada, Sebastián Reyes-Cerpa

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Salmonid rickettsial septicemia (SRS), caused by Piscirickettsia salmonis, has been the most severe health concern for the Chilean salmon industry. The efforts to control P. salmonis infections have focused on using antibiotics and vaccines. However, infected salmonids exhibit limited responses to the treatments. Here, we developed a poly (D, L-lactide-glycolic acid) (PLGA)-nanosystem functionalized with Atlantic salmon IgM (PLGA-IgM) to specifically deliver florfenicol into infected cells. Polymeric nanoparticles (NPs) were prepared via the double emulsion solvent-evaporation method in the presence of florfenicol. Later, the PLGA-NPs were functionalized with Atlantic salmon IgM through carbodiimide chemistry. The nanosystem showed an average size of ~380–410 nm and a negative surface charge. Further, florfenicol encapsulation efficiency was close to 10%. We evaluated the internalization of the nanosystem and its impact on bacterial load in SHK-1 cells by using confocal microscopy and qPCR. The results suggest that stimulation with the nanosystem elicits a decrease in the bacterial load of P. salmonis when it infects Atlantic salmon macrophages. Overall, the IgM-functionalized PLGA-based nanosystem represents an alternative to the administration of antibiotics in salmon farming, complementing the delivery of antibiotics with the stimulation of the immune response of infected macrophages.

Original languageEnglish
Article number1658
JournalNanomaterials
Volume14
Issue number20
DOIs
StatePublished - Oct 2024

Keywords

  • Atlantic salmon macrophages
  • florfenicol
  • nanosystem
  • Piscirickettsia salmonis
  • PLGA

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