TY - JOUR
T1 - Simple Fluorescence Sensing Approach for Selective Detection of Fe3+Ions
T2 - Live-Cell Imaging and Logic Gate Functioning
AU - Rasin, Puthiyavalappil
AU - Manakkadan, Vipin
AU - Vadakkedathu Palakkeezhillam, Vishnunarayanan Namboothiri
AU - Haribabu, Jebiti
AU - Echeverria, Cesar
AU - Sreekanth, Anandaram
N1 - Publisher Copyright:
© 2022 The Authors. Published by American Chemical Society.
PY - 2022/9/20
Y1 - 2022/9/20
N2 - A pyrene-based fluorescent chemosensor APSB [N-(pyrene-1-ylmethylene) anthracen-2-amine] was designed and developed by a simple condensation reaction between pyrene carboxaldehyde and 2-aminoanthracene. The APSB fluorescent sensor selectively binds Fe3+ in the presence of other metal ions. Apart from this, APSB shows high selectivity and sensitivity toward Fe3+ ion detection. The detection limit for APSB was 1.95 nM, and the binding constant (Kb) was obtained as 8.20 × 105 M-1 in DMSO/water (95/5, v/v) medium. The fluorescence quantum yields for APSB and APSB-Fe3+ were calculated as 0.035 and 0.573, respectively. The function of this fluorescent sensor APSB can be explained through the photo-induced electron transfer mechanism which was further proved by density functional theory studies. Finally, a live-cell image study of APSB in HeLa cells was also carried out to investigate the cell permeability of APSB and its efficiency for selective detection of Fe3+ in living cells.
AB - A pyrene-based fluorescent chemosensor APSB [N-(pyrene-1-ylmethylene) anthracen-2-amine] was designed and developed by a simple condensation reaction between pyrene carboxaldehyde and 2-aminoanthracene. The APSB fluorescent sensor selectively binds Fe3+ in the presence of other metal ions. Apart from this, APSB shows high selectivity and sensitivity toward Fe3+ ion detection. The detection limit for APSB was 1.95 nM, and the binding constant (Kb) was obtained as 8.20 × 105 M-1 in DMSO/water (95/5, v/v) medium. The fluorescence quantum yields for APSB and APSB-Fe3+ were calculated as 0.035 and 0.573, respectively. The function of this fluorescent sensor APSB can be explained through the photo-induced electron transfer mechanism which was further proved by density functional theory studies. Finally, a live-cell image study of APSB in HeLa cells was also carried out to investigate the cell permeability of APSB and its efficiency for selective detection of Fe3+ in living cells.
UR - http://www.scopus.com/inward/record.url?scp=85138762272&partnerID=8YFLogxK
U2 - 10.1021/acsomega.2c03718
DO - 10.1021/acsomega.2c03718
M3 - Article
AN - SCOPUS:85138762272
SN - 2470-1343
VL - 7
SP - 33248
EP - 33257
JO - ACS Omega
JF - ACS Omega
IS - 37
ER -