TY - JOUR
T1 - Insights into neutralizing antibody responses in individuals exposed to SARS-CoV-2 in Chile
AU - Beltrán-Pavez, Carolina
AU - Riquelme-Barrios, Sebastián
AU - Oyarzún-Arrau, Aarón
AU - Gaete-Argel, Aracelly
AU - González-Stegmaier, Roxana
AU - Cereceda-Solis, Karina
AU - Aguirre, Adam
AU - Travisany, Dante
AU - Palma-Vejares, Ricardo
AU - Barriga, Gonzalo P.
AU - Gaggero, Aldo
AU - Martínez-Valdebenito, Constanza
AU - Le Corre, Nicole
AU - Ferrés, Marcela
AU - Balcells, María Elvira
AU - Fernandez, Jorge
AU - Ramírez, Eugenio
AU - Villarroel, Franz
AU - Valiente-Echeverría, Fernando
AU - Soto-Rifo, Ricardo
N1 - Publisher Copyright:
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
PY - 2021/2/12
Y1 - 2021/2/12
N2 - Chile has one of the worst numbers worldwide in terms of SARS-CoV-2 positive cases and COVID-19-related deaths per million inhabitants; thus, characterization of neutralizing antibody (NAb) responses in the general population is critical to understanding of immunity at the local level. Given our inability to perform massive classical neutralization assays due to the scarce availability of BSL-3 facilities in the country, we developed and fully characterized an HIV-based SARS-CoV-2 pseudotype, which was used in a 96-well plate format to investigate NAb responses in samples from individuals exposed to SARS-CoV-2 or treated with convalescent plasma. We also identified samples with decreased or enhanced neutralization activity against the D614G spike variant compared with the wild type, indicating the relevance of this variant in host immunity. The data presented here represent the first insights into NAb responses in individuals from Chile, serving as a guide for future studies in the country.
AB - Chile has one of the worst numbers worldwide in terms of SARS-CoV-2 positive cases and COVID-19-related deaths per million inhabitants; thus, characterization of neutralizing antibody (NAb) responses in the general population is critical to understanding of immunity at the local level. Given our inability to perform massive classical neutralization assays due to the scarce availability of BSL-3 facilities in the country, we developed and fully characterized an HIV-based SARS-CoV-2 pseudotype, which was used in a 96-well plate format to investigate NAb responses in samples from individuals exposed to SARS-CoV-2 or treated with convalescent plasma. We also identified samples with decreased or enhanced neutralization activity against the D614G spike variant compared with the wild type, indicating the relevance of this variant in host immunity. The data presented here represent the first insights into NAb responses in individuals from Chile, serving as a guide for future studies in the country.
UR - http://www.scopus.com/inward/record.url?scp=85101007844&partnerID=8YFLogxK
U2 - 10.1126/sciadv.abe6855
DO - 10.1126/sciadv.abe6855
M3 - Article
C2 - 33579701
AN - SCOPUS:85101007844
SN - 2375-2548
VL - 7
JO - Science Advances
JF - Science Advances
IS - 7
M1 - eabe6855
ER -
